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產(chǎn)品目錄

普諾賽HT-29 (人結(jié)腸癌細(xì)胞)

品名: 普諾賽HT-29 (人結(jié)腸癌細(xì)胞)
型號(hào): CL-0118
  • 產(chǎn)品詳情
  • 規(guī)格參數(shù)

產(chǎn)品信息

名稱HT-29 (人結(jié)腸癌細(xì)胞) (STR鑒定正確)
別稱HT 29; HT29
背景描述HT-29細(xì)胞是由J·Fogh在1964年用移植培養(yǎng)方法和含15%FBS的F12培養(yǎng)液從原發(fā)性腫瘤分離的;近來,已建株的培養(yǎng)細(xì)胞用含相同血清McCoy's5A培液培養(yǎng)。HT-29細(xì)胞在裸鼠中致瘤,也能在類固醇處理的地鼠中致瘤。HT-29細(xì)胞合成IgA、CEA、蛋白綁定TGF-β的分泌成分和粘液素等。
種屬
年齡(性別)女;44歲
組織來源結(jié)直腸腺癌
生長(zhǎng)特性貼壁細(xì)胞
細(xì)胞形態(tài)上皮細(xì)胞樣
細(xì)胞類型腫瘤細(xì)胞
腫瘤類型腸癌細(xì)胞
生物安全等級(jí)1
生長(zhǎng)培養(yǎng)基McCoy’s 5A10% FBS1% P/S
推薦傳代比例1:3-1:4
推薦換液頻率2~3次/周
倍增時(shí)間~36-60 hours
凍存條件凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度:液氮
培養(yǎng)條件氣相:空氣,95%;CO2,5%溫度:37℃
致瘤性Yes, in nude mice; forms well differentiated adenocarcinoma consistent with colonic primary (grade I); tumors also form in steroid treated hamsters.
受體表達(dá)情況human adrenergic alpha2A, urokinase receptor (u-PAR), vitamin D (moderate expression), urokinase receptor (u-PAR); vitamin D (moderate expression), human adrenergic alpha2A
基因表達(dá)情況secretory component of IgA; carcinoembryonic antigen (CEA); transforming growth factor beta binding protein; mucin, myc+; ras+; myb+; fos+; sis+; p53+; abl -; ros -; src -, Blood Type A; Rh+; HLA A1, A3, B12, B17, Cw5, HT-29 cells are negative for CD4, but there is cell surface expression of galactose ceramide (a possible alternative receptor for HIV).
保藏機(jī)構(gòu)ATCC; HTB-38 BCRJ; 0111 CCLV; CCLV-RIE 0960 DSMZ; ACC-299 ECACC; 91072201
STR鑒定
  • STR位點(diǎn)信息

    AmelogeninX
    CSF1PO11,12
    D2S133819,23
    D3S135815,17
    D5S81811,12
    D7S82010
    D8S117910,16
    D13S31711,12
    D16S53911,12
    D18S5113
    D19S43314
    D21S1129,30
    FGA20,22
    PentaD11,13
    PentaE14,16
    TH016,9
    TPOX8,9
    vWA17,19
    D6S104312,14
    D12S39118.3,21
    D2S44111


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