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普諾賽NCI-H292 (人肺癌細(xì)胞(淋巴結(jié)轉(zhuǎn)移))

品名: 普諾賽NCI-H292 (人肺癌細(xì)胞(淋巴結(jié)轉(zhuǎn)移))
型號(hào): CL-0167
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產(chǎn)品信息

名稱NCI-H292 (人肺癌細(xì)胞(淋巴結(jié)轉(zhuǎn)移)) (STR鑒定正確)
別稱H292; H-292; NCI-HUT-292; Hut292; NCIH292
背景描述NCI-H292細(xì)胞源自肺支氣管黏液上皮樣癌的淋巴結(jié)轉(zhuǎn)移灶;先用成份限定的培養(yǎng)基分離得到,然后換成含血清的培養(yǎng)基培養(yǎng)。培養(yǎng)條件下,NCI-H292細(xì)胞保留了黏液上皮的特性,可從其超微結(jié)構(gòu)的表現(xiàn)和多種鱗狀上皮分化標(biāo)記的表達(dá)而判斷。NCI-H292細(xì)胞支持HBV的生長(zhǎng),左旋多巴脫羧酶陰性。NCI-H292細(xì)胞可以作為篩選模型,將人類(lèi)亞基因組片段轉(zhuǎn)染入細(xì)胞來(lái)研究HBV及其自身基因在病毒性肝炎和肝癌發(fā)生中的作用。NCI-H292細(xì)胞角蛋白、波形蛋白、黏液洋紅染色陽(yáng)線,但神經(jīng)絲三聯(lián)蛋白陰性。
種屬
年齡(性別)女;32歲
組織來(lái)源肺粘膜上皮細(xì)胞癌;肺
生長(zhǎng)特性貼壁細(xì)胞
細(xì)胞形態(tài)上皮細(xì)胞樣
細(xì)胞類(lèi)型腫瘤細(xì)胞
腫瘤類(lèi)型肺癌細(xì)胞
生物安全等級(jí)1
生長(zhǎng)培養(yǎng)基RPMI-164010% FBS1% P/S
推薦傳代比例1:3-1:4
推薦換液頻率2~3次/周
倍增時(shí)間~48 hours
凍存條件凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度:液氮
培養(yǎng)條件氣相:空氣,95%;CO2,5%溫度:37℃
致瘤性Yes, in nude mice; tumors histologically resemble the original biopsy specimen and retain mucoepidermoid features.
基因表達(dá)情況keratin; vimentin,The cells retain their mucoepidermoid characteristics in culture as determined by their ultrastructure and expression of multiple markers of squamous differentiation. TANK cells show reactivity with human alloantisera specific for the HLA A1, A3, A10, A19, B12, B17, B22 and B40 cross-reactive groups.
保藏機(jī)構(gòu)ATCC; CRL-1848
STR鑒定
  • STR位點(diǎn)信息

    AmelogeninX
    CSF1PO10
    D2S133821,24
    D3S135816
    D5S81813
    D7S82010
    D8S117911,14
    D13S31711,12
    D16S5399,13
    D18S5116
    D19S43312,13.2
    D21S1128
    FGA22,26
    PentaD9
    PentaE13,15
    TH018
    TPOX8,11
    vWA16,17
    D6S104311,12
    D12S39116,20
    D2S44111,12


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