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產(chǎn)品目錄

普諾賽M-07e (人巨細胞白血病細胞)

品名: 普諾賽M-07e (人巨細胞白血病細胞)
型號: CL-0686
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產(chǎn)品信息

名稱M-07e (人巨細胞白血病細胞) (STR鑒定正確)
別稱M-07E; M-O7e; M07-e; M07e; Mo7e; MO7e; M07E; MO7E
背景描述Established from the peripheral blood of a 6-month-old girl with acute megakaryoblastic leukemia (AML M7) at diagnosis in 1987; subline of the growth factor-independent cell line M-07; M-07e cells respond proliferatively to GM-CSF, IFN-alpha, IFN-beta, IFN-gamma, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-alpha, TPO; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to outgrowth of independent cells. Exome and RNA sequence data are available
種屬人類
年齡(性別)女性,6個月
組織來源外周血
生長特性懸浮細胞
細胞形態(tài)淋巴母細胞樣
細胞類型腫瘤細胞
腫瘤類型白血病細胞
生物安全等級1
生長培養(yǎng)基RPMI-164010% FBS+8ng/mL GM-CSF+1% P/S
推薦傳代比例5×10^5-1×10^6cells/mL
推薦換液頻率2~3次/周
倍增時間~40小時
凍存條件凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度:液氮
培養(yǎng)條件氣相:空氣,95%;CO2,5%溫度:37℃
抗原表達情況CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, HLA-DR -
注意事項該細胞為懸浮細胞,請注意離心收集細胞懸液;請勿直接倒掉細胞培養(yǎng)液。
保藏機構(gòu)DSMZ; ACC-104
STR鑒定
  • STR位點信息

    AmelogeninX
    CSF1PO9,10
    D2S133819,25
    D3S135816,19
    D5S81811
    D7S82011
    D8S117913,16
    D13S31710,11
    D16S53911
    D18S5115,20
    D19S43314,15
    D21S1130,32.2
    FGA18,22
    PentaD9
    PentaE12,14
    TH016,8
    TPOX8
    vWA16,18
    D6S104313,19
    D12S39123,24
    D2S44114


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