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產(chǎn)品目錄

普諾賽SU-DHL-4 (人B淋巴瘤細(xì)胞)

品名: 普諾賽SU-DHL-4 (人B淋巴瘤細(xì)胞)
型號: CL-0692
  • 產(chǎn)品詳情
  • 規(guī)格參數(shù)

產(chǎn)品信息

名稱SU-DHL-4 (人B淋巴瘤細(xì)胞) (STR鑒定正確)
別稱SUDHL4; Sudhl4; SUDHL-4; Sudhl-4; SuDHL 4; SUD-4; SUD4; SU4; Stanford University-Diffuse Histiocytic Lymphoma-4; DHL-4; DHL4
背景描述Established from the peritoneal effusion of a 38-year-old man with B-NHL (diffuse large cell, cleaved cell type; originally described as "diffuse histiocytic lymphoma") in 1975; cell line carries EZH2 Y641S mutation; assigned to GCB-like lymphoma subtype (germinal center B-cell). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)
種屬人類
年齡(性別)男性,38歲
組織來源腹腔積液
生長特性懸浮細(xì)胞
細(xì)胞形態(tài)淋巴母細(xì)胞樣
細(xì)胞類型腫瘤細(xì)胞
腫瘤類型淋巴瘤細(xì)胞
生長培養(yǎng)基RPMI-164010% FBS1% P/S
推薦傳代比例5×10^5-1×10^6cells/mL
推薦換液頻率2~3次/周
倍增時間~40小時
凍存條件凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度:液氮
培養(yǎng)條件氣相:空氣,95%;CO2,5%溫度:37℃
基因表達(dá)情況IgG+; Kappa+; IgM-; IgA-; IgD-; Lambda-; This cell line has relatively high expression levels of Bax; Bak; AIF; high caspase-9 activity.
注意事項該細(xì)胞為懸浮細(xì)胞,請注意離心收集細(xì)胞懸液;請勿直接倒掉細(xì)胞培養(yǎng)液。
保藏機(jī)構(gòu)ATCC; CRL-2957 DSMZ; ACC-495
STR鑒定
  • STR位點(diǎn)信息

    AmelogeninX,Y
    CSF1PO12
    D2S133817,19
    D3S135815,16
    D5S81811,12
    D7S8208,11
    D8S117913
    D13S31712
    D16S53911,13
    D18S5114,16
    D19S43314,15.2
    D21S1129,31
    FGA19,21
    PentaD9,14
    PentaE7,12
    TH016,9.3
    TPOX9,11
    vWA18,19
    D6S104311
    D12S39118,20
    D2S44111,14


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