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產(chǎn)品目錄

普諾賽bEnd.3 [BEND3] (小鼠腦微血管內(nèi)皮細胞)

品名: 普諾賽bEnd.3 [BEND3] (小鼠腦微血管內(nèi)皮細胞)
型號: CL-0598
  • 產(chǎn)品詳情
  • 規(guī)格參數(shù)

產(chǎn)品信息

名稱bEnd.3 [BEND3] (小鼠腦微血管內(nèi)皮細胞) (種屬鑒定正確)
別稱bEND.3; b.End3; Bend.3; bEnd3; brain-derived Endothelial cells.3
背景描述The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen
種屬小鼠
年齡(性別)6周齡
組織來源大腦皮層
生長特性貼壁細胞
細胞形態(tài)上皮細胞樣
細胞類型轉(zhuǎn)化細胞系
生物安全等級1
生長培養(yǎng)基DMEM10% FBS1% P/S
推薦傳代比例1:4-1:6
推薦換液頻率2~3次/周
倍增時間~24-32 hours
凍存條件凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度:液氮
培養(yǎng)條件氣相:空氣,95%;CO2,5%溫度:37℃
抗原表達情況ICAM-1 +; VCAM-1 +; MAdCAM-1 +
基因表達情況von Willebrand factor,ICAM-1 +; VCAM-1 +; MAdCAM-1 +,The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL).,The expression of Peyer's Patch high endothelial receptor for lymphocytes, the mucosal vascular addressin (MAdCAM-1) and E-selectin can be induced on bEnd
保藏機構(gòu)ATCC; CRL-2299 BCRC; 60515 ECACC; 96091929
STR鑒定
  • STR位點信息

    18-318
    6-711,12
    5-514
    X-126
    1-213
    7-129
    8-116
    1-110
    19-213
    15-319.3,20.3
    12-117
    6-417
    4-218.3
    3-214
    2-19
    13-116.2
    11-214
    17-212


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